Fig 2: Evaluation of the MIT effect on the regulators of mitochondrial dynamics in the brain using semi-quantitative Western blot analysis. The brain tissue samples were collected from the TG and NTG control mice and TG mice treated with MIT for 3 months. (A) Representative immunoblots showing the expression of MFF, TFAM, and CKMT1 proteins in brain tissues collected from individual mice. (B) Densitometric analysis of the relative expression of MFF, TFAM, and CKMT1 proteins in brain tissues obtained from different study groups. There was a significant difference in the expression of MFF and CKMT1 proteins in NTG control (p < 0.05 for both) and TG MIT treatment (p < 0.01 and p < 0.001 for MFF and CKMT1, respectively) groups in comparison to the TG control group. The data are expressed as mean ± SD (N = 6 for the NTG control group, N = 9 for the TG control group, and N = 10 for the TG MIT treatment group). SD is denoted by error bars. * p < 0.05, ** p < 0.01, and *** p < 0.001 were compared between NTG control mice, TG control mice, and MIT-treated TG mice using a one-way ANOVA followed by a Tukey’s post hoc multiple comparison test.